合浦珠母贝卵子电击脉冲参数的初步研究     

喻达辉  贾晓平  陈素文  李夏  朱嘉濠 《海洋科学》2005,29(8):15-19

运用电脉冲转基因仪对合浦珠母贝卵子进行电脉冲处理以优化电穿孔法转基因的电击参数。实验中调制百分比：100％；脉冲间隔：1．0s：电极距离：2mm；脉冲个数：5个，参数保持不变。用不同的电脉冲参数组合（脉冲电压100-400V、脉冲持续时间0．5～3．0ms、脉冲频率10～50kHz，共28个组合）对卵子电击，然后加入精子进行授精，统计各组受精率和孵化率，以受精率和孵化率达到50％以上为衡量指标。结果表明，合浦珠母贝卵子对低脉冲频率和高电压非常敏感，当频率低于20kHz或脉冲电压高于400V时对卵子损伤较大。脉冲持续时间对其影响不大，适应范围较宽。优化的转基因电击脉冲参教组合为：脉冲电压1.0kV／cm，脉冲持续时间20ms，脉冲频率50kHz，或者脉冲电压1．5kV／cm，脉冲持续时间1．5ms，脉冲频率50kHz。  相似文献   

A time-varying parameter model of a body oscillating in pitch     

A.P. McCabe  G.A. Aggidis  T.J. Stallard   《Applied Ocean Research》2006,28(6):359-370

Among the assumptions upon which linear time-invariant models of floating bodies are based is that the body motions are so small that any change in the body’s angular position can be disregarded. However, it is often a major design requirement of a wave energy conversion device that the response amplitude is large, thereby invalidating one of the assumptions of the linear model. In particular, the immersed geometry of a body undergoes considerable variation when it is moved in pitch. With regard to this we investigate the difference in performance between a quasi-linear model in which the change of immersed surface is modelled by time-varying parameters and a basic linear model in which the immersed surface is time-invariant. The time-varying parameter model is realized by interpolation between the appropriate parameter values of a set of linear time-invariant (LTI) models derived for the different immersed surfaces that occur at discrete body displacements. It is shown that the responses predicted using the time-varying parameter model are closer to those measured experimentally than those of a standard frequency-domain model. Particular improvement occurs when the responses are large, such as at or near the resonance frequency. A problem which may limit the general use of the model is also discussed.  相似文献   

海洋水色卫星遥感器特性参数探讨     

于连生 《海洋技术学报》2001,20(2):76-80

为了评价海洋水色卫星遥感器特性参数的优劣，确定仪器的计量特性及其使用价值，准确的理解和科学的定义仪器的特性参数是非常重要的。作者曾有幸参加了海洋水色卫星的立项论证工作，本文就作者的一些工作体会，对海洋水色卫星遥感器的特性参数作初步探讨。  相似文献   

西太平洋暖池区海—气通量计算分析   总被引：4，自引：0，他引：4  

徐天真  陈伯海 《中国海洋大学学报(自然科学版)》1993,(Z2)

用J.Launiaimen和T.Vihma提出的近地面层湍流通量计算方法,对我国在1992年11月至1993年2月TOGA—COARE—IOP实验中所获资料计算处理。得出所在站位的海一气间显效、潜热及动量通量。指出西大平洋暖池海区游热通量与显效通量之比为10.14:1;风速大于8m/s后各通量随风速的变化率明显增加;动量与热量的块体通量系数Cd和Ce,h随风速变化有相似的规律;Monin—Obukhov大气稳定度参数Z/L与△T/U_(10)之间有较好的统计关系。  相似文献   

水产饲料膨化机设计刍论     

徐钟良  陈启发 《台湾海峡》2001,20(Z1):207-211

据开发应用水产饲料膨化机实践，初步论述了膨化机生产能力与动力匹配，螺杆设计参数长径比，物料在腔内滞留时间，膨化腔设计及其温度调控，并提出改进设计的见解.  相似文献   

Baroclinic Buoyancy-Inertia Joint Stability Parameter     

Hideo Kawai 《Journal of Oceanography》2005,61(2):235-246

At present, the barotropic buoyant stability parameter has been derived from a vertical virtual displacement of a water parcel. The barotropic inertial stability parameter in the eccentrically cyclogeostrophic, basic current field was derived in 2003 from a horizontal cross-stream virtual displacement of a parcel. By expressing acceleration of a parcel due to a virtual displacement, which is arbitrarily sloping within a vertical section across the basic current, in terms of natural coordinates, we derived the vertical component of baroclinic buoyant stability parameter B ₂ ², the horizontal component of baroclinic inertial stability parameter I ₂ ², the baroclinic joint stability parameter J ², its buoyant component B ² and its inertial component I ². B ² is far greater than I ₂ ², and when neglecting relative vorticity except for vertical shear, a downward convex curve of J ² plotted against the slope of a virtual displacement follows a trend of B ² curve. If a parcel displaces along a horizontal surface or an isopycnal surface, however, B ² vanishes, and J ² becomes equal to I ². Actual parcel is apt to displace not only along the bottom slope, but also along the sea surface and an isopycnal interfacial surface, which is approximately equivalent to an isentropic surface, preferred by lateral mixing and exchange of momentum. Such actual displacement makes B ² vanishing, and grants I ² an important role. The present analysis of I ² examining effects due to curvature and horizontal and vertical shear vorticities are useful in deepening our understanding of baroclinic instability in actual oceanic streams.  相似文献   

波群作用于垂直桩柱的峰力统计性质     

徐立论  陈志军  焦海英 《中国海洋大学学报(自然科学版)》1994,(1)

研究具有窄谱和Ｗｅｉｂｕｌｌ波高分布的波群对非线性桩柱系统作用力的统计性质。求得了桩桩的波浪峰力的各种特征值及其比值。指出这些数值不仅是阻力一惯性力参数ｂＨ的函数，也随着波群因子而变化。本文模式更具广泛性。文中给出了一系列计算图表，可从理论计算波群作用于桩柱的波浪峰力。  相似文献   

ASM1中污水水质参数与化学计量系数的估测     

王波 《海岸工程》2002,21(4):6-12

简要介绍了活性污泥1号模型中14个污水水质参数与5个化学计量系数的估测方法和典型取值。  相似文献   

Sediment movement under unidirectional flows: an assessment of empirical threshold curves   总被引：1，自引：0，他引：1  

D. Paphitis 《Coastal Engineering》2001,43(3-4)

A selection of threshold data sets from previous investigations is used to re-examine, selectively, some of the empirical curves that are most commonly used for the prediction of sediment threshold. Simple analytical formulae are derived describing single line curves for mean threshold values and envelopes for the initial movement of discrete particles and the commencement of mass sediment transport phases of the critical condition.The complexity of factors governing the prevailing conditions at threshold, together with the randomness of events, limits deterministic solutions; however, the curves provide an approach that can be used readily in practical applications. In contrast, the envelope presentation is more of a stochastic approach to the definition of threshold. It recognises that there is no definitive threshold condition under which a particular particle size can be displaced, but rather that a range of threshold values exist.The empirical threshold relationships were derived on the basis of a relatively large amount of data. However, the sources utilised have interpreted the ‘critical condition’ in various ways, implying that the formulae must be treated with caution in applications.  相似文献   

Bowen数和东中国海Bowen数分布     

张淮水  汪园祥  楼顺里 《中国海洋大学学报(自然科学版)》1988,(3)

本文讨论Bowen数的意义、功能和计算法。同时,依据多年水文气象实测资料作统计,计算出东中国海的Bo值。其结果绘制成1月至12月的月平均分布图,从而对本海域的Bo分布特点作详细分析介绍。  相似文献